OBJECTIVES: . The study assessed the cost-effectiveness (CE) of a medical device, an urine collection/transportation system containing or not preservative (UCTS) versus the traditional open device (TOD). The evaluated UCTS is used to collect and transport urine specimens, reducing contamination, and providing more accurate test results to establish UTI presence in non-pregnant women from the Mexican Institute of Social Security (IMSS) perspective.  METHODS: . The CE analysis was based in a decision model, considering a time horizon of two weeks after urine samples were collected. The number of correctly diagnosed UTI was the effectiveness measure. Data was obtained from available published studies reporting contamination rates. To calculate the effectiveness measure of the diagnoses process, a hypothetical cohort of 500 patients was simulated and positive/negative predictive values of the laboratory tests involved in the process were considered for adjustment.  Direct medical costs were included and obtained from IMSS available data. CER and ICER were calculated to establish the cost-effectiveness of both medical devices. Univariate and multivariate sensitivity analysis, using pricing discounts and effectiveness were performed.  RESULTS: . Costs of UTI diagnosis in the hypothetical cohort using the UCTS were USD1,907.40 compared to USD 2,163.2 with the TOD. The number of correctly diagnosed UTI for each alternative was 25% and 51% respectively. CER were USD 3,742.80/correctly diagnosed UTI and USD 8,597.79/correctly diagnosed UTI with the TOD. ICER analyses suggest that UCTS is a cost effective alternative, saving USD 4,854.99 for each correctly diagnosed UTI. The sensitivity analysis confirmed the robustness of the modeled parameter estimates. CONCLUSIONS: . The results from the analyses indicates that UCTS (containing or not preservative) provides a highly cost-effective alternative compared with TOD for collecting, transporting and improving the quality of urine specimens in non-pregnant women from the payer perspective in Mexico.