The burden of dengue is significant in Brazil. Between 2002 and 2010 approximately four million probable dengue cases were reported, with significant epidemics in 2002, 2008 and 2010. It is expected that a dengue vaccine, currently under phase 3 clinical trials, will reduce the number of cases, costs of the disease and costs of dengue prevention and control programs, in particular during epidemic peaks. OBJECTIVES: Estimate the costs of dengue control program (PMCD) in the municipality of Goiânia-GO, Brazil. METHODS: We conducted a retrospective analysis, considering epidemic (October/2009-April/2010), and endemic (May-September/2010) periods of dengue transmission. The public healthcare system perspective was considered. We considered the following cost components: human resources, training, infrastructure, equipment and office supplies, transportation, personal protective equipment (PPE), field and laboratory equipment, insecticides and social mobilization, considering capital and recurrent costs. Capital costs were estimated for both transmission periods combined. RESULTS: The total and monthly recurring costs of the PMCD during the epidemic period was R$8.307.590 (US$4.988.345) and R$1.400.819 (US$841.131) respectively; and in the endemic period was R$ 5.848.678 (US$ 3.511.876) and R$950.619 (US$570.805), with human resources being responsible for the majority of costs in both periods (83 and 86%, respectively). Capital costs for both periods were R$683,315 (U.S.$410,301), with a high cost share for transportation (80%) and infrastructure (13%) components. The Municipal health department was responsible for 83% of total costs, followed by state (13%), and federal (3%) levels. CONCLUSIONS: Although the estimated costs of the PMCD is under-estimated, we demonstrate a significant incremental costs (R$3.050,211 or U.S.$1.831.518) during the epidemic period. Our results can provide inputs for cost-effectiveness studies of dengue vaccines, which are important to support decision making regarding its introduction into public health programs. Keywords: dengue, cost of dengue, program costs, economic evaluation.