OBJECTIVES: To evaluate the knowledge towards the etiology and treatment of Tuberculosis (TB) among TB patients in North Eastern Libya METHODS: Face to face interview with all TB Patients in 2009 was conducted at Kuwaifia and Shahat Chest Hospitals in North Eastern Libya. Questionnaire included questions related to the knowledge about transmission, diagnosis; risk factors, treatment and prevention of TB. A total of 140 patients participated in this study. Knowledge scores ranged from zero (minimum score) to 23 (maximum score). All data was analyzed using SPSS version 15.00®. Inferential statistics were used as whenever appropriate. P value of less than 0.05 was considered significant.RESULTS: Majority of the respondents were Libyans (n=92, 65.7%). Fifty-five (39.3%) of the respondents were males, and up to 90 subjects (64.3%) were urban residents. Almost all of the respondents have heard about TB.  Nearly (n =70, 50.0%) shared a thought that the main source of their knowledge regarding TB were health workers, followed by television (n=63, 45.0%), and family members (n=34, 24.3%). Libyans have significantly better knowledge (12.26±4.33) than non Libyans (7.85±4.83, t = 30.12, P <0.001). In addition those respondents with tertiary educations scored significantly higher knowledge score (15.7± 4.2) compared to those of intermediate (15.3 ±3.8) and illiterate (9.4 ± 3.5), [F=30.12, P = 0.001]. Moreover, respondents from urban areas were found to have better knowledge (12.06 ± 3.82) than rural residents (8.38± 4.92, t = 20.0), (p=<0.001). Whilst, in term of level of income those with high income (12.37± 3.79) have significantly better knowledge than low income (8.00±4.85), [F=5.5, p<0.001].  CONCLUSIONS: The level of knowledge about TB among TB patients was a low, Therefore, there is a need for massive health education campaign to be undertaken by policy makers in order to improve TB patients’ knowledge towards TB