OBJECTIVES: The present study was conducted to find out the impact of mother’s education on immunization status of children. METHODS:  The study was a cross-sectional questionnaire based survey performed in different immunization centers of South Punjab, Pakistan. Total 424 parents with 12-23 months old children were interviewed about the vaccination status of children. Vaccination coverage was assessed by both vaccination card and parent's recall methods. SPSS version 20.0 was used to analyze the data. Appropriate statistics was applied to summarize and analyze the data. RESULTS:  Complete immunization coverage was seen in 69.5% children while 30.5 % children were found incompletely immunized. Knowledge regarding immunization was also assessed and majority of parents were found with an average level of knowledge and mean knowledge score was 9.13+2.90. However a significant association (p=0.001) was observed between the maternal education and vaccination status of children. CONCLUSIONS: Results of the study concluded that complete vaccination trend was more common in educated mothers as compared to illiterate mothers. So vaccination coverage can be improved by educating and providing awareness to parents about immunization.