OBJECTIVES:  To assess the impact of an educational intervention on community residents’ knowledge and attitudes towards antibiotics in Jelutong District, Penang, Malaysia. METHODS:  A single cohort pre/post-intervention study was conducted among the community residents between 30^th January and 30^th April 2016. Using convenience sampling, 70 residents (aged ≥18 years) were invited to participate in an educational intervention that included a talk, video presentation and the distribution of an antibiotic booklet about the responsible use of antibiotics. Pre-intervention, the participants were asked to complete a validated, pre-tested self-administered questionnaire. Then, they were asked to complete the same questionnaire immediately post-intervention, at 1 month and 3 months follow-up. The questionnaire consisted of nine statements assessing their knowledge of antibiotics and six statements to assess their attitudes towards antibiotics. The primary outcome measures were a post-intervention increase of their antibiotic knowledge and attitude scores. Data was analysed using the Statistical Package for the Social Sciences version 22.0 and was presented as mean ± standard deviation. RESULTS:  Sixty eight out of 70 residents participated in the study (response rate = 97.14%), but six of them (8.82%) dropped-out at 3 months follow-up. Their knowledge scores were 2.50±0.92, 8.24±0.74, 7.85±0.78 and 7.23±0.42 at the pre-intervention, immediately post-intervention, at 1 month and 3 months follow-up, respectively (p<0.001). Their attitude scores were 2.32±0.72, 5.82±0.38, 5.50±0.50 and 5.13±0.34 at the pre-intervention, immediately post-intervention, at 1 month and 3 months follow-up, respectively (p<0.001). CONCLUSIONS: The educational intervention significantly increased the knowledge and positively changed the attitudes of the community residents towards antibiotics. Hence, further initiatives should include the scaling up of the intervention to promote the responsible use of antibiotics among community residents in Malaysia.