OBJECTIVES: The Study aimed to explore the knowledge and perception of Regulatory pharmacists regarding Drug Regulation in Pakistan. METHODS: A cross sectional descriptive study was conducted to evaluate the Knowledge and perception of Regulatory Pharmacist regarding Drug Regulation in Pakistan from November - June 2016. The study was carried out in Pakistan major cities of Pakistan where regulatory pharmacists were practicing. Study tool comprised of Demographics and six main domains including; Amendment in Existing Drug regulation, Procedural flaws and delay, Lack of knowledge and Training, Lack of budget and other facilities, Political Influence and security Issues, Poor communication and administration between different bodies. All analyses were done by using SPSSv20 RESULTS: Result showed that maximum respondents 155 (57.2%) age ranges between 32-41 years. Most of respondents 185 (68.3%) were male having B-Pharmacy. Majority 123 (45.4%) of respondents were Drug inspectors. maximum of respondents 148(54.6%) were disagreeing in that Drug act1976 is complete and comprehensive for drug regulation in Pakistan and provide complete and comprehensive leverage to Provincial Government to made rules. Majority of respondents 119(43.9%) were disagree that Drug act provide proper procedure for all regulatory activities. While 95(35.1%) were disagree in that Academic learning provides proper knowledge to Regulatory Pharmacist during study. Most of pharmacists 145(53.5%) were agree that they have security issues when they go for inspection. Majority of respondents 138(50.9%) were disagree that information which is present by one authority is not disseminate to other. CONCLUSIONS:  Study concluded that regulatory pharmacists in Pakistan had mixed perceptions towards drug regulations. The findings showed that misapprehensions about certain sections/subsections of drug law persist among regulatory pharmacists. It is high time that the policy makers and regulatory pharmacists should sit together for mutual consensus development regarding the discrepancies in the current drug act