OBJECTIVES:  The willingness to donate blood in Hungary decreased gradually in the last decades. The aim of the study is to assess the practice, knowledge and attitude of donors’ towards blood donation. METHODS:  A cross-sectional, quantitative study with non-probability, convenience sampling was performed in Kecskemét between 2016. 07. 01 – 2016. 09. 31. All responders were between 18-65 years, and had a residence in Bács-Kiskun county (N=200). Responders who filled out less than 70% of the questionnaire ware excluded. The questionnaire used in the study had questions about socio-demographic data; health status; locations, amount, frequency of blood donations; knowledge and attitude towards blood donations. For analysis we used SPSS 22.0. Besides descriptive statistics (mean, median, mode, standard deviation, frequency) we calculated Spearman correlation, Kruskall-Wallis test, Mann-Whitney U test, linear regsression, χ²-test and ANOVA (p<0,05). SPSS 22.0 was used for calculation. RESULTS:  Mean age of donors was 41.43 years (SD=11.27, min=18 years, max=65 years). Mean score of the knowledge test was 14.44±1.58 points (min=11, max=19). Socio-demographic data do not have an affect on knowledge, nor regularity of blood donations (p>0.05). Fear of needle, pain and infection shows a negative correlation with the number of donations. Low haemoglobin level is the most frequent reason to be excluded from donation. There is no connection between knowledge level and attitude towards blood donation (p>0.05). CONCLUSIONS:  Based on our results we conclude the following: good cooperation with the donors are important; interests of young people must be increased; opportunity of donation on weekends and afternoons (after morning shift schedules) must be given; maintain people’s motivation and expand knowledge about blood donation is necessary.