OBJECTIVES: The present study was conducted to evaluated knowledge and awareness regarding postmenopausal syndrome (PMS) among the females in Quetta, Pakistan. METHODS: A cross-sectional study was conducted among the females aged 30 years and above from January to September 2016. Knowledge regarding postmenopausal syndrome was assessed by a self-administrated structured questionnaire contain different questions related to PMS. Descriptive analysis was used to demonstrate the characteristics of the study population. Inferential statistics (Kruskal Wallis tests, p < 0.05) were used to assess the significance among study variables. RESULTS: A total of 550 distributed questionnaires, 447 were returned (with response rate of 81%). Mean age of the respondents was 38.06 ±6.194 years. Mean of the total knowledge score was 12.77±3.910. Fifty-three percent had adequate knowledge regarding postmenopausal syndrome and 47.0% had poor knowledge regarding postmenopausal syndrome.73.5% of the respondents were aware about the age of menopause. None of the demographic characteristics has statistical significant association with the mean knowledge scores of the study participants.  CONCLUSIONS: The study respondents had poor level of knowledge about postmenopausal syndrome Healthcare professionals should give information to females about post-menopausal syndrome so that, the knowledge could be improved.