OBJECTIVES:  The study aimed to assess the awareness among medical and pharmacy students of different colleges and universities of Pakistan toward Swine flu (Influenza AH1N1). METHODS:  A descriptive, cross sectional study design was adopted to conduct the study. A self-administered questionnaire was administered in 500 students of both urban and rural community of Pakistan during January 2016 to October2016. Respondents were selected by using convenient sampling method. Data was analyzed using IBM SPSS version 20. Descriptive statistics were applied to evaluate the data and chi square test was used to check association between dependent and independent variables. RESULTS:  Among the participants, 221(44.2%) were male and 279(55.8%) were female. Almost all respondents 455(91.0%) had knowledge about origin of disease, genotype: H1N1 268(53.6%), H1N2 198(39.6%), mode of transmission: human 221(44.2%), pigs 298(55.8%) and preventive measures 475(95%) of swine fluParticipants were having less knowledge about vaccination 285(57%), lab screening, and availability of facilities to practitioner and patients by government regarding swine flu. CONCLUSIONS:  Knowledge regarding swine flu pandemic was good among participants but need further improvement as well. Government should focus on providing isolated ward facility, special diagnostic and treatment facility to swine flu patients. Government should focus on facilitating mask availability to practitioners as protective measure