OBJECTIVES: South Africa has one of the highest incidence rates of tuberculosis (TB) in the world. Healthcare workers (HCWs) are at a particularly high risk of developing active TB compared to the general population due to their occupational exposures. Using a Best-Worst Scaling (BWS) choice experiment, this study aimed to systematically quantify and compare the TB screening preferences of physicians, nurses and healthcare administrators working in the publicly funded healthcare system in South Africa.  METHODS: Three focus groups and three key informant interviews were conducted to determine relevant attributes and attribute levels. Participants answered 12 choice tasks. Conditional logit modelling of BWS data allowed the estimation of coefficients for 15 attribute levels relative to a reference level allowing the determination of utility values RESULTS: One hundred-and-five HCWs completed the questionnaire, including 65 (62%) nurses, 21(20%) administrators, and 19 (18%) physicians. Analysis revealed that all HCWs preferred testing at their occupational health clinics compared to other clinics in the community. Administrators had the greatest preference for screening at no cost (Mean: 6.20, SE: 0.44). Physicians had the strongest preference to not wait for their testing (4.97, SE: 0.40) compared to all other attribute levels. Administrators and nurses had a strong preference to ensure the confidentiality of their tests (5.91, SE: 0.44, and 5.49, SE: 0.23, respectively), while this was less preferred for physicians (3.54, SE: 0.39).  Nurses and physicians were indifferent to the HCW conducting the test, whereas administrators had a strong preference to be tested by a physician (5.58, SE: 0.43). CONCLUSIONS: There is considerable variation in TB screening preferences amongst physicians, nurses and healthcare administrators in South Africa. Attention to heterogeneity in preferences will optimize utilization of screening programs amongst this high-risk population.