OBJECTIVES: The world’s worst outbreak of Ebola occurred in 2014 with 21,296 cases and 8,429 deaths reported in total. The first case of Ebola was recorded on 30^th September by Centers for Disease Control and Prevention (CDC) in the US. Pharmacists can play an important role in treating Ebola patients. The aim of this study is to assess knowledge and attitude about Ebola among U.S. pharmacy students. METHODS: This was a cross sectional survey study. An Ebola questionnaire was distributed among third year pharmacy students in a private university in the U.S. before and after delivering an educational Ebola seminar based on the CDC and World Health Organization Ebola fact sheet N103. The questionnaire comprised of 33 questions was divided into three components: demographics (3), Ebola knowledge (25) and attitude about Ebola (5). Paired t test and McNemar test were employed using SPSS version 21.  RESULTS: A total of 103 pharmacy students participated in the survey. The study population had a highly significant increase in Ebola related knowledge about species (p<0.001), incubation period (p<0.001), diagnosis (p<0.001), vaccination (p<0.05), treatment (p<0.001), complications (p<0.001) and immunity (p<0.01). Also, a significant number of study participants had a positive attitude about treating Ebola patients and believed that Ebola patients should be kept isolated (p<0.05) and communities should actively participate in preventing the spread of Ebola (p<0.05).  CONCLUSIONS: Students, upon becoming PharmD professionals can apply the knowledge acquired through this study for effectively treating patients. Focused seminars are a valuable tool to improve student awareness of infectious disease.