OBJECTIVES: Given the increasing need to promote rational use of drugs, the World Health Organization (WHO) encourages regular drug utilization evaluation (DUE) in healthcare facilities to provide insight into patterns, quality, and determinants of drug use. This study profiled anti-infectives to understand their extent of use. METHODS: The study was a cross sectional retrospective analysis of patients prescription records from June to December 2016, based on WHO’s recommended drug use indicators. Data were analyzed using descriptive statistics. RESULTS: Of the 624.0 patients prescription records studied, 329.0 (55.0%) were females. The mean age was 42.0±4.6 years while the mean number of drugs per prescription was 2.94±0.6 against the reference value of 1.6 - 1.8. Out of 1833.0 drugs prescribed, 646.0 (32.2%) were anti-infectives. The number of drugs prescribed by generic was 975.0 (53.2%) against the 100.0% benchmark recommended by WHO. This comprised of antibacterial drugs 475.0 (73.5%) against a standard reference of 20.0 – 26.8, antivirals 4.0 (0.6%), antifungal drugs 16 (2.5%), antiprotozoal 120.0 (18.6%), and anthelmintics 31.0 (4.8%). Of the anti-infectives prescribed, quinolones were the most prescribed 213.0 (44.8%) followed by amoxicillin+Clavulanic acid 52.0 (11.0%). The most prescribed quinolone was ciprofloxacin 146.0 (30.7%) followed by ofloxacin 65.0 (13.7%). The most prescribed antiprotozoal agent was metronidazole 91.0 (75.8%). The anti-infectives prescribed as injectables were 188.0 (29.1%). CONCLUSIONS: The high use of anti-infectives was an indication of high prevalence of infections and irrational use of drugs. Other indications for irrationality in drug use include poor generic prescribing, higher number of drugs per prescription, and high proportion of injectables. They predispose patients to high cost of medicines and non-adherence considering catastrophic spending in developing countries driven by rise in pharmaceutical expenditures. This baseline study has provided fundamental data for further studies in promoting the rational use of anti-infectives in Nigeria.