OBJECTIVES: To determine the Prevalence and risk factors of Hepatitis B & C among the  Barbers and their regular shaving clients  in the Hyderabad Barber Shops of Pakistan . METHODS:  A cross sectional study was conducted to determine the sero-prevalence of Hepatitis-B virus (HBV) and Hepatitis-C virus (HCV) among barbers and their clients in Hyderabad Sindh Pakistan and to assess their knowledge, attitude and practices regarding these two viruses and their mode of transmission. Sampling was done by using a-2-stage sampling techniques. A close ended and open ended multi-country questionnaire was designed to collect data from 715 participants (186 Barbers and 529 Clients). Blood samples were withdrawn after obtaining an informed consent and were tested for HBV and HCV markers by Chromatography, enzyme-lined immunosorbent assay (ELISA) and polymerase chain reaction (PCR). RESULTS: The mean age was 28.47±9.7 years in both groups of Barbers (n-186) and Clients (n=529). Among both groups, the sero-prevalence of HBV and HCV was 5.7% and 14.4%, respectively. Clients knew about hepatitis B and C viruses while barbers were not quite aware. The knowledge about the route of transmission was poor among barbers and good among clients. Half of the respondents in both groups knew about hepatitis B vaccination and only 15% were vaccinated. Sixty percent of the barbers claimed disinfecting the instruments between clients and (88.9%) claimed using of new blades. During actual observation of practices, only 28% disinfected instruments between clients and 62% used new blades for each client. CONCLUSIONS: There is some awareness among barbers and clients about hepatitis B and C viruses but poor knowledge about the mode of transmission. This warrants conducting health education campaigns to increase awareness about these two blood borne viruses and the risk factors associated with their transmission particularly at barbers’ shop and to implement interventions to prevent spreading Hepatitis.