OBJECTIVES: Quinolnes are broad-spectrum antibacterials with activity against many organisms resistant to aminoglycosides, antipseudomonal penicillins, and third generation cephalosporins. Any compromise in their utilization could be catastrophic in tailoring bacteria treatments to desired outcomes. This study assessed the rationality of quinolones utilization based on World Health Organization benchmark. METHODS: The study was a retrospective cross-sectional descriptive study of all eligible prescriptions with quinolones that met the inclusion criteria. Prescriptions were assessed based on prescription and utilization indicators. The prescriptions analyzed covered two years period from January 2013 to December 2014. Study lasted from July 2015 to May 2016. Data were summarized using descriptive statistics. RESULTS: The total number of drugs prescribed was 5229 while the mean number of drugs per prescription was 4±2.38. Drugs prescribed from the essential drug list were 4952 (95%) and generic prescriptions were 3254 (62%). The age group with the highest quinolone use was those between 18- 40 years while the least was those below 18 years. The number of antibiotics prescribed was 2280 (44%) and fraction of quinolones in antibiotics prescribed was 480 (21%). The most prescribed quinolone was Ciprofloxacin 279 (58%), followed by Ofloxacin 104 (22%). The least prescribed was Germifloxacin 3(0.6%). The most prescribed dosage form was oral tablets and suspensions 99%, parenteral 0.6% and otics 0.4%. The antibiotics most prescribed together with the quinolones were Ceftriaxone 4% and Cefuroxime 2%. The mean duration of quinolones use in prescriptions was 9±3.4 days. CONCLUSIONS: The study indicated irrational use of quinolones characterized by polypharmacy and brands prescription, which is associated with high cost of medications in a region where majority of people live below $ 2 USD per day. This is one of the predisposing factors to medication non-adherence and therapeutic failure. It further endangers the already threatened compounds to resistance by mutant strains.