OBJECTIVES: Present study is conducted to determine the trend and use of antibiotics among the post-operative patients of tertiary care hospital. METHODS: The present study was designed as retrospective study.  The data was obtained from the records of tertiary care hospital (Bolan Medical complex Hospital). The data was gathered randomly from the record for the period between July to October, 2015. Information regarding antibiotic use along patient related data was obtained. The collected data was reviewed, coded, verified and statistically analyzed using SPSS. RESULTS: Data from the patients,  who has been prescribed antibiotic after surgery was obtained. a total of 325 records were include in the study. Appendicitis was most common cause of admission (N= 124, 40.6%). Most of the patients (N= 209, 68.5%) hospitalized for maximum of 4 days in hospitals after surgery. Most commonly prescribed antibiotics were Ceftriaxone, Metronidazole and Gentamicin. Cases of multiple antibiotic in treatment were high, in which triple antibiotics containing treatments were leading (N=220, 72%) followed by double antibiotics containing treatments (N= 85, 28%).  CONCLUSIONS: The study revealed that there was high number of antibiotics prescribed to the post operative patients irrationally, as there is no evidence for the prescription of these antibiotics. Antibiotics are prescribed in multiple forms.  There is strong need of a local Guidelines to be established for use of antibiotics rationally.