OBJECTIVES: To investigate factors affecting nurses’ working motivation on job satisfaction in public and private hospitals in Pafos. METHODS: The study included all hospitals in Pafos, with 410 nurses out of which a random sample was collected from February to March 2013. Two generic, self-administered, instruments have been used. The first questionnaire refers to working motivation in terms of recognition, responsibility, personal development, job interest, working relationships etc, developed by Everard και Morris (1999). The second instrument refers to job satisfaction by Spector (1985). Both instruments score 1: “agree/satisfied very much” to 4 “disagree/dissatisfied very much”. Correlation analysis was performed to identify the relationship between working motivation and job satisfaction. Also, multivariate regression analysis with stepwise method was conducted between overall job satisfaction and various working motivation dimensions. RESULTS: