OBJECTIVES: Preparation of Voriconazole (VCZ) solid lipid nanoparticles (SLNs) for effective ocular delivery for the treatment of fungal keratitis. METHODS: SLNs were prepared by solvent emulsification technique using Compritol (lipid), Pluronic F-68 (surfactant) and sodium taurocholate (co-surfactant). Characterization of SLNs was performed by size measurement, in-vitro release, ex-vivo corneal permeation studies and in-vitro antifungal activity. RESULTS: Particle sizes were found in the range of 150-300 depending upon lipid/S_mix ratio with good zeta potential. Entrapment efficiency of SLNs was found between 40-60% with sustained in vitro drug release (>70% in 12h). The ex-vivo corneal permeation studies exhibited good ocular permeation of VCZ from SLNs. Ex-vivo study also supports good ocular permeation of VCZ from SLNs when compared with drug suspension. Further, in-vitro antifungal activity exhibited the potential of VCZ SLNs. CONCLUSIONS: The sustained release property with good corneal permeation of VCZ from SLNs encourages its application for in-vivo studies and hence could be proposed as an effective carrier for ophthalmic administration.