OBJECTIVES: Trends in the use of herbal medications continue to rise. Pharmacists have an opportunity to provide consumers with evidence based information.  This study examined pharmacy student’s knowledge regarding herbal medication from courses and the associated outcomes towards attitude formation.  METHODS: In a cross-sectional prospectively designed study a survey was self-administered to second, third and final year doctorate of pharmacy students at a University with diverse student body. The questionnaire included four sections: attitude, education, knowledge and demographic. Pre-validated instruments were used to measure attitude towards herbal medications using a five-point likert scale while knowledge was measured using a true-false scale. Descriptive statistics were used to summarize data and multiple logistic regression was conducted to assess the effect of pharmacy student’s herbal medication knowledge towards attitude formation while controlling for age, race, sex, year of study, work experience and herbal medication related courses. RESULTS: Of 206 students, 159 completed the survey (response rate of 77.18%).  Mean age of participants was 26 (±3.2) years with 65.41% female and 81.13% had previous pharmacy work experience.  The average score on the herbal knowledge was 9.28±1.80 (maximum score of 15). Students with higher knowledge had positive attitude towards herbal medications (OR=1.26; 95% CI=1.01-1.57). Students with work experience had positive attitude towards herbal medications (OR=2.93; 95% CI=1.05-8.14).  However, 81% students believed that they did not have sufficient knowledge of herbal medications and 91% students reported that they needed more information. CONCLUSIONS: Higher knowledge leads to a positive attitude towards herbal medications among pharmacy students indicating that they may recommend such products to patients. Evaluating the role of such recommendations to improve patients health outcomes is the direction for the future.