OBJECTIVES: Patient-relevant endpoints play an important role in Health Technology Assessment (HTA). There is a need to prioritize these endpoints according to patients’ preferences. Our aim was to investigate how glaucoma patients prioritize different aspects of their treatment including patient-relevant endpoints. METHODS: The study included a feasibility test and the completion of a specific questionnaire at the ophthalmology clinic of Bonn. Patients rated the importance of different aspects of glaucoma treatment by a pairwise comparison. Relative weights were generated for each aspect by Analytic Hierarchy Process (AHP), a multi-criteria decision analysis method using matrix algebra. . Additionally the EQ-5D was applied to stratify the patients into subgroups according to their stated utility. RESULTS: The AHP yielded the following results (Weight, Mean, SD, CI) by downwards order: 1. Autonomy (0.394, 0.371 ± 0.145, 0.311 - 0.431), subdivided in household chores (0.239, 0.275 ± 0.258, 0.168 - 0.381) and outdoor mobility (0.761, 0.725 ± 0.258, 0.619 - 0.832). 2. Reading and seeing details (0.229, 0.212 ± 0.123, 0.161 - 0.263). 3. Darkness and glare (0.153, 0.165 ± 0.111, 0.119 - 0.211).  4. Peripheral vision (0.089, 0.085 ± 0.058, 0.061 - 0.109). 5. Side effects (0.088, 0.115 ± 0.131, 0.060 - 0.168), and 6. Treatment-related burden (0.047, 0.052 ± 0.06, 0.027 - 0.076). The observed inconsistency reached a consistency ratio of 0.04 and did not exceed the limit of 0.1. Subgroup analyses according to the EQ-5D stratification showed adaptation effects and loss aversion. CONCLUSIONS: AHP can be used in HTA to give a quantitative dimension to patients’ preferences for treatment aspects. Preference elicitation could provide important information at various stages of HTA and challenge opinions on the importance of treatment aspects or endpoints.