OBJECTIVES The aim of this study was to translate and validate a Brazilian version of the “Prolapse – Quality Of Life Questionnaire “ (P-QOL) as a specific instrument to assess the severity of symptoms and their impact in the quality of life of Brazilian women with genital prolapse. METHODS Sixty-five patients (45 with symptomatic and 20 with asymptomatic pelvic organ prolapse), were enrolled from the outpatient clinic of the Uroginecology and Vaginal Surgery Section of the Gynecology Department of the Federal University of São Paulo (UNIFESP). At first, we translated the P-QOL into Brazilian Portuguese language following international methodological recommendations. Because of language and cultural differences we performed cultural, structural, conceptual, and semantic adaptation on the P-QOL, so that patients were capable to completely understand the questions. All patients answered P-QOL twice on the same day with an interval of 30 minutes, applied in face-to-face interviews by two different interviewers. After 7 to 15 days, by phone interview, P-QOL was applied again. The reliability assessed using Cronbachxs alpha and validity was assessed comparing symptom scores between affected and asymptomatic women and comparing symptom scores with objective prolapse stages. RESULTS The results showed that the Brazilian Portuguese version of P-QOL has very good psychometrics properties. The total scores for each P-QOL domain were significantly different between symptomatic and asymptomatic women (p<0.05). All items achieved a Cronbachxs alpha greater than 0.70 showing moderate to good inter-observer reliability. The test-retest reliability confirmed a highly significant correlation between the total scores for each domain. CONCLUSIONSP-QOL was cross-culturally adapted and validated for Brazilian women with genital prolapse, showing good reliability and validity. The Brazilian Portuguese version of P-QOL is a good option for Brazilian researchers to evaluate the quality of life in women with pelvic organ prolapse.