Objective: To determine antimicrobial resistance profiles for Enterococcus in 14 tertiary-level hospitals in Bogotá, Colombia Methods: Time series analyses were performed, as well as descriptive analyses of Enterococcus faecalis and Enterococcus faecium in hospitals belonging to the Bogotá Bacterial Resistance Control Group from January 1, 2001-January 1, 2007. We identified the presence and species of enterococcus according to anatomical site Results: During that period, a total of 5770 strains of Enterococci faecalis and 1259 of Enterococcus faecium were analyzed. Enterococcus was found in>20% of isolates from blood samples and abdominal fluid in critical care units (SUCI) and in >10% of samples from non-critical care services (SNUCI) in tertiary-level hospitals in Bogotá. Ampicillin was active against Enterococcus faecalis strains in Critical care units (SUCI) and other services (SNUCI), but >50% of E.faecium strains in SNUCI were resistant. Vancomycin-resistant strains occurred in >2% of E.faecalis strains identified in SUCI and >8% in SNUCI. The resistance trend in E.faecium to vancomycin in SNUCI was towards the low in the six years studied Conclusion: This study confirms the worldwide trend towards an increase in infections due to Enterococcus. The emerging pattern of antimicrobial resistance among such isolates is alarming