OBJECTIVES: To ascertain the scoring and to assess the psychometric properties of the Eye-Drop Satisfaction Questionnaire (EDSQ). METHODS: The EDSQ was developed to assess the satisfaction and compliance of patients with glaucoma treatment. The hypothesised model content of the questionnaire resulting from patient and clinician interviews organised 43 items into six domains: Patients characteristics, Treatment characteristics, Patient-clinician relationship, Patient experience, Patient-treatment interaction, and Patient knowledge. The EDSQ was included in a observational, multicenter study in France in patients treated for glaucoma. The scoring of the EDSQ was investigated by testing several possible structures, resulting from the hypothesised model/Factor Analysis. Psychometric properties of the retained structure were assessed: internal consistency reliability (Cronbach’s alpha) and construct validity (multitrait analysis). Distributional features (missing data, ceiling and floor effects) of the  EDSQ scores were scrutinised. RESULTS: Analyses were performed with 169 patients who had completed more than half of the EDSQ items. The retained structure, resulting from Principal Component Analysis, included six domains: Concern about treatment (5 items), Concern about disease (2 items), Satisfaction with patient-clinician relationship (5 items), Positive beliefs (3 items), Treatment convenience (3 items), and Declared compliance (3 items). Items not included in the domains were kept for separate analysis. Cronbach’s alpha of the domains was greater than 0.7 for five domains and ranged from 0.65 to 0.90. The structure offered good item convergent and item divergent validity (range of item-scale correlation: 0.36-0.82). Moderate ceiling effect was observed for the Satisfaction with patient-clinician relationship score (20% at maximum score), reflecting good relationships.  An important ceiling effect was also observed for the Declared compliance score, with patients declaring high levels of compliance (49% at maximum score). CONCLUSIONS:The scoring of the EDSQ was developed and the questionnaire was shown to have satisfactory psychometric properties.