OBJECTIVES: The study aimed to examine the role of generational influences on the use and attitudes towards Complementary and Alternative Medicine (CAM). METHODS: A non-experimental cross-sectional study design was employed. The study subjects comprised of students from a diverse university. The generations were classified as: first if the study subject was the first one to leave another country to settle/study in US, second if the study subject have parents to leave another country first while not being a US born child, third if the study subject have parents to leave another country first and the subject is US born child, fourth if the subject has US born parents but immigrant grandparents and fifth if the subject has US born grandparents. Students’ attitude, use and practice towards CAM were examined using a 5-point Likert scale.  Descriptive analysis, chi-square tests and analysis of variance were carried out to test the research objectives. RESULTS: From the total 400 completed surveys, majority of the respondents were females (55.7%) and Asians/Pacific Islanders (28%), the mean age being 22.6 (± 5.0) years. Almost 63% of participants had used CAM in the past and 36% were currently practicing CAM. First generation students (21.5%) were more frequent users of CAM, followed by fifth generation students (18.5%). Post Hoc/ Scheffe test revealed significant difference in attitude towards CAM between generations 1-2 (mean difference 2.951), generations 1-3 (mean difference 0.25) and generations 1-4 (mean difference 0.25). First generation (10.25%) and fifth generation (10.75%) students’ use of CAM was most influenced by their generation values (p<0.0195). CONCLUSIONS: Generation influenced attitude towards CAM as well as their practice. First generation students had a greater role in CAM use as compared to other generations, indicating that first generation students take time to embed the use of conventional medicine as the domestic individuals.